bio rad cfx opus 384 (Bio-Rad)
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bio rad cfx opus 384
Bio Rad Cfx Opus 384, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 8624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+cfx+opus+384/SsoAdvanced+Universal+SYBR+Green+Supermix/bio_rxiv__64898__2026__03__14__711737-201-15-15
Average 99 stars, based on 8624 article reviews
Bio Rad Cfx Opus 384, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 8624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+cfx+opus+384/SsoAdvanced+Universal+SYBR+Green+Supermix/bio_rxiv__64898__2026__03__14__711737-201-15-15
Average 99 stars, based on 8624 article reviews
bio rad cfx opus 384 - by Bioz Stars,
2026-10
99/100 stars
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Real-time Polymerase Chain Reaction:Article Title: A microRNA diagnostic biomarker for amyotrophic lateral sclerosis. Article Snippet: For determination of sample signal, we measured six endogenous miRNAs: miR-142–3p, miR-451a, miR-23a-3p, miR-30c-5p, miR-103a-3p and miR-191-5p.28 qPCR of miRNA using SYBR green We conducted qPCR for all QC, target and reference miRNA using Qiagen miRCURY LNA miRNA SYBR PCR Assays (cat. no. 339306; Supplementary Table 2 for GeneGlobe IDs) and the miRCURY LNATM SYBR Green PCR Kit (Qiagen, cat. no. 339347) according to the manufacturer’s instructions (Supplementary Table 3) and as described previously.27 Briefly, we diluted cDNA 1/30 into nuclease-free water and used 3 μL in each 10 μL qPCR reaction. .. We automated the pipetting of master mix (7 μL) and samples (3 μL) into 384-well plates using the Opentrons OT-2 liquidhandling robot using a custom protocol written in Python. qPCR was conducted on the Article Title: Cytotoxic CD4 + T cells in the bone marrow compromise healthy ageing by enhancing granulopoiesis Article Snippet: Quantitative PCR (qPCR) primers (Invitrogen-ThermoFisher) were designed using Primer-BLAST (NCBI; sequences are detailed below). .. A total of 10 ng of cDNA was used for quantitative PCR in a total volume of 10 μl with GoTaq® qPCR Master Mix (Promega) and specific primers, on a Article Title: miRNA extracted from extracellular vesicles is a robust biomarker of amyotrophic lateral sclerosis. Article Snippet: .. qPCR for target and reference gene miRNAs (Supplementary Table 2) was conducted using Qiagen miRCURY LNA miRNA SYBR PCR Assays #339306 and the miRCURY LNATM SYBR Green PCR Kit (g339347, Lot #76901890, SYBR Green Lot #N822.3) on the Article Title: L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint Article Snippet: .. qPCR for all quality control (QC), target and reference gene miRNAs was conducted using Qiagen miRCURY LNA miRNA SYBR PCR Assays (cat. no. 339306 and see Supplemental Data 5-T1 for GeneGlobe IDs for QC assays and Supplemental Data 5-T2 for targets and reference genes) and the miRCURY LNATM SYBR Green PCR Kit (Qiagen cat. no. 339347) according to the manufacturer’s instructions and as described previously [ ]. qPCR was conducted on the Polymerase Chain Reaction:Article Title: A microRNA diagnostic biomarker for amyotrophic lateral sclerosis. Article Snippet: For determination of sample signal, we measured six endogenous miRNAs: miR-142–3p, miR-451a, miR-23a-3p, miR-30c-5p, miR-103a-3p and miR-191-5p.28 qPCR of miRNA using SYBR green We conducted qPCR for all QC, target and reference miRNA using Qiagen miRCURY LNA miRNA SYBR PCR Assays (cat. no. 339306; Supplementary Table 2 for GeneGlobe IDs) and the miRCURY LNATM SYBR Green PCR Kit (Qiagen, cat. no. 339347) according to the manufacturer’s instructions (Supplementary Table 3) and as described previously.27 Briefly, we diluted cDNA 1/30 into nuclease-free water and used 3 μL in each 10 μL qPCR reaction. .. We automated the pipetting of master mix (7 μL) and samples (3 μL) into 384-well plates using the Opentrons OT-2 liquidhandling robot using a custom protocol written in Python. qPCR was conducted on the Article Title: miRNA extracted from extracellular vesicles is a robust biomarker of amyotrophic lateral sclerosis. Article Snippet: .. qPCR for target and reference gene miRNAs (Supplementary Table 2) was conducted using Qiagen miRCURY LNA miRNA SYBR PCR Assays #339306 and the miRCURY LNATM SYBR Green PCR Kit (g339347, Lot #76901890, SYBR Green Lot #N822.3) on the Article Title: L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint Article Snippet: .. qPCR for all quality control (QC), target and reference gene miRNAs was conducted using Qiagen miRCURY LNA miRNA SYBR PCR Assays (cat. no. 339306 and see Supplemental Data 5-T1 for GeneGlobe IDs for QC assays and Supplemental Data 5-T2 for targets and reference genes) and the miRCURY LNATM SYBR Green PCR Kit (Qiagen cat. no. 339347) according to the manufacturer’s instructions and as described previously [ ]. qPCR was conducted on the SYBR Green Assay:Article Title: miRNA extracted from extracellular vesicles is a robust biomarker of amyotrophic lateral sclerosis. Article Snippet: .. qPCR for target and reference gene miRNAs (Supplementary Table 2) was conducted using Qiagen miRCURY LNA miRNA SYBR PCR Assays #339306 and the miRCURY LNATM SYBR Green PCR Kit (g339347, Lot #76901890, SYBR Green Lot #N822.3) on the Article Title: L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint Article Snippet: .. qPCR for all quality control (QC), target and reference gene miRNAs was conducted using Qiagen miRCURY LNA miRNA SYBR PCR Assays (cat. no. 339306 and see Supplemental Data 5-T1 for GeneGlobe IDs for QC assays and Supplemental Data 5-T2 for targets and reference genes) and the miRCURY LNATM SYBR Green PCR Kit (Qiagen cat. no. 339347) according to the manufacturer’s instructions and as described previously [ ]. qPCR was conducted on the other:Article Title: CD8⁺ T cells induce interstrand crosslinking-associated DNA damage in neurons Article Snippet: Nuclei were counterstained with DAPI (Life Technologies, cat #D1306) at 1 μg/ml, then coverslips were mounted on slides with Prolong Gold (Life Technologies, cat #P36930), and allowed to cure for at least 24 hours before imaging with a Stellaris 8 fluorescent confocal microscope. Control:Article Title: L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint Article Snippet: .. qPCR for all quality control (QC), target and reference gene miRNAs was conducted using Qiagen miRCURY LNA miRNA SYBR PCR Assays (cat. no. 339306 and see Supplemental Data 5-T1 for GeneGlobe IDs for QC assays and Supplemental Data 5-T2 for targets and reference genes) and the miRCURY LNATM SYBR Green PCR Kit (Qiagen cat. no. 339347) according to the manufacturer’s instructions and as described previously [ ]. qPCR was conducted on the |